Hi Nadeem,
Thank you for your response...
Telnet connection is working fine from our Sap Server.
As i have mentioned that the the SAPOSS rfc is working fine with the other 3 Sap Instances which has been installed in the same server, getting issue only with one instance.
Also we have assigned correct permission in the route tab for this server.
Suggest me how to move forward.
Regards,
Krishna.M