Hi Kaushik,
As you activated the connection pooling for new connections, please make sure the keep-alive timeout of the back end system is greater value than the keep-alive timeout of the Web dispatcher. You can find more details in following link.
http://help.sap.com/saphelp_nw73/helpdata/en/48/996b4bec0973e9e10000000a42189b/content.htm
Also did you restart the service after changing webdisp pfl ?
Thanks